U.S. Department of Agriculture, Agricultural Research Service (USDA-ARS): Converting Alaska Fish By-Products Into Value-Added Ingredients and Products

This project is being conducted with the collaboration of the USDA Agricultural Research Service, the University of Alaska Fairbanks, School of Fisheries and Ocean Sciences, and the Hagerman Fish Culture Experiment Station, University of Idaho.

ALASKA RESEARCH PLAN 2011-2012

This research plan is to fulfill “SUBOBJECTIVE: 3.1. for fiscal year 2011-2012 is to evaluate novel feed additives, made from seafood processing byproducts as growth enhancers as written in the 5 year Research Plan.

The overall goal is to evaluate the nutritional value of testes meals and fractions extracted from testes meal in the Pacific white shrimp or the Pacific threadfin (tropical marine fish.) The Alaskan fisheries harvest produce large amounts of mature fish testes that are underused. Previous data generated from threadfin has shown that dried testes meal can replace up to 57% of standard whtefish meal in a feed formulation containing 25% soybean meal without adverse effect on frowth performance. The testes meal also showed potential to improve protein utilization by the threadfin. Fractions extracted from fish testes meal are also of interest as potential feed ingredients or additives to stimulate growth in cultured fish and shrimp.  

In this research plan, we will plan further studies to investigate: 1) The effect of supplementation of extracted fractions of lipid, nucleotide and small basic proteins on growth of the Pacific white shrimp or the Pacific threadfin; and 2) present and publish data from this project and pasts projects listed below.

TITLE:

Identify factors in byproduct meals that stimulate growth in fish and shrimp

SPECIFIC GOAL:

The overall goal is to evaluate the nutritional value of testes meals and their enriched fractions in Pacific white shrimp and the Pacific threadfin (tropical marine fish).

EXPRIMENTAL DESIGN:

The overall goal is to evaluate the nutritional value of a fish lipid extraction, one for nucleotide fraction and one for a small basic protein fraction. in Pacific white shrimp (Litopanaeus vannamei) or Pacific threadfin (Polydactylus sexfilis). The Alaska salmon harvest produces large amounts of mature fish testes that are not used in feed. Preliminary data indicate the dried testes had high digestibility and performed well in shrimp growth trials. Enriched fractions of fish testes meal/oil are of interest as potential alternative ingredients for the aquaculture feed industry. 

In this study, we will plan (3) three growth trials, to test the hypothesis. The specific objective is to study the effect of a fish lipid extract, a nucleotide fraction and a small basic protein fraction on the growth performance of the Pacific threadfin or the Pacific white shrimp in a plant protein based diets. Evaluation will be based on growth and effect of body composition of the fish or shrimp fed after 8 weeks of feeding with graded levels of fish lipid extract, a nucleotide fraction and a small basic protein fraction. Each dietary treatment will be fed to triplicate groups, in a randomized experimental design. Shrimp (average size ca. 1 g/animal) or moi (average 3g/fish) will be maintained in a clear flow through water system. Animals will be weighed initially, every two weeks and at the end of the trial. Experimental diets will incorporate the ingredients to be tested into partial replacement of the fish meal and plant protein diets on an equal protein basis. The amount of fish oil added will be adjusted to make the diets approximately isocaloric.

All data will be analyzed for statistical significance using ANOVA. When ANOVA reveals a significant treatment effect, an appropriate “post-hoc” test will be employed to identify significant differences between treatments.

Growth Trial

The specific objective here is to:

Investigate the testes extracted fractions: a). lipids, b). nucleotides, c). small basic proteins on the growth performance of shrimp or fish.

Evaluation will be based on growth and effect of body composition of the fish or shrimp fed after 8 weeks of feeding with graded levels of fish testes protein meal or the extracted fractions. Each dietary treatment will be fed to triplicate groups, in a randomized experimental design. Pacific white shrimp (average size ca. 0.5-­‐1 g/animal) and threadfin (average 3g/fish) will be maintained in a clear flow through water system. Animals will be weighed initially, every two weeks and at the end of the trial. Experimental diets will incorporate the ingredients to be tested into partial replacement of the fishmeal and plant protein diets on an equal protein basis. The amount of fish oil, if any, added to formulations will be adjusted to make the diets approximately isocaloric.

Data Management

All data will be analyzed for statistical significance using ANOVA. When ANOVA reveals a significant treatment effect, an appropriate post-hoc test will be employed to identify significant differences between treatments.